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axioimager m2 fluorescence microscope  (Carl Zeiss)


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    Carl Zeiss axioimager m2 fluorescence microscope
    Axioimager M2 Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioimager+m2+system/software+zen/bio_rxiv__2025__07__11__663895-203-8-7
    Average 90 stars, based on 1 article reviews
    axioimager m2 fluorescence microscope - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Nail-associated mesenchymal cells contribute to and are essential for dorsal digit tip regeneration.
    Article Snippet: Counterstaining with Hoechst 33258 (Sigma) or DAPI (Thermo) was performed to visualize the cell nuclei. .. Digital images were acquired and analyzed with either a Quorum Spinning-Disk confocal microscope system using Volocity acquisition software (PerkinElmer, Waltham, MA), or a Zeiss LSM900 with an Airyscan 2 detector using Zen 3.4 software or a Zeiss AxioImager M2 system with an X-Cite 120 LED light source and a C11440 Hamamatsu camera using Zen acquisition software (Thornwood, NY), or a Zeiss AxioImager M2 system with an Illuminator microLED light source and an Axiocam 712 mono camera using Zen 3.1 software. ..

    Software:

    Article Title: Nail-associated mesenchymal cells contribute to and are essential for dorsal digit tip regeneration.
    Article Snippet: Counterstaining with Hoechst 33258 (Sigma) or DAPI (Thermo) was performed to visualize the cell nuclei. .. Digital images were acquired and analyzed with either a Quorum Spinning-Disk confocal microscope system using Volocity acquisition software (PerkinElmer, Waltham, MA), or a Zeiss LSM900 with an Airyscan 2 detector using Zen 3.4 software or a Zeiss AxioImager M2 system with an X-Cite 120 LED light source and a C11440 Hamamatsu camera using Zen acquisition software (Thornwood, NY), or a Zeiss AxioImager M2 system with an Illuminator microLED light source and an Axiocam 712 mono camera using Zen 3.1 software. ..

    Article Title: Specific and comprehensive genetic targeting reveals brain-wide distribution and synaptic input patterns of GABAergic axo-axonic interneurons
    Article Snippet: Confocal imaging was performed on a Zeiss LSM 780 (CSHL St. Giles Advanced Microscopy Center) using ×10, ×20, or ×63 objectives. .. Imaging for grayscale overview images was performed on a Zeiss Axioimager M2 System equipped with MBF Neurolucida Software (MBF) using ×5, ×10, or ×20 objectives. ..

    Article Title: Dynamic regulation of autophagy during Semliki Forest virus infection of neuroblastoma cells
    Article Snippet: The secondary antibody, anti-rabbit AlexaFluor488, was diluted with blocking buffer and incubated at room temperature for 40 min. Coverslips were mounted using FluoroshieldTM with a DAPI mounting medium (Sigma-Aldrich, UK). .. Imaging of multiple z -axis positions was performed using a Zeiss AxioImager M2 system, and images were deconvoluted using the Zeiss Zen software deconvolution module. .. For quantification of LC3-positive punctae, images of only the DyLight 550 channel were selected at random and spots were counted in ImageJ.

    Article Title: Dynamic regulation of autophagy during Semliki Forest virus infection of neuroblastoma cells.
    Article Snippet: The secondary antibody, anti- rabbit AlexaFluor488, was diluted with blocking buffer and incubated at room temperature for 40 min. Coverslips were mounted using FluoroshieldTM with a DAPI mounting medium (Sigma- Aldrich, UK). .. Imaging of multiple z- axis positions was performed using a Zeiss AxioImager M2 system, and images were deconvoluted using the Zeiss Zen software deconvolution module. ..

    Article Title: Nail-associated mesenchymal cells contribute to and are essential for dorsal digit tip regeneration.
    Article Snippet: .. Stitched images were acquired on a Zeiss AxioImager M2 system and ImageJ analysis software (Bethesda, MD) was used to trace outlines of the PDGFRa -positive regenerated region. ..

    Article Title: Specific and comprehensive genetic targeting reveals brain-wide distribution and synaptic input patterns of GABAergic axo-axonic interneurons
    Article Snippet: Confocal imaging was performed on a Zeiss LSM 780 (CSHL St. Giles Advanced Microscopy Center) using 10x, 20x, or 63x objectives. .. Imaging for grayscale overview images was performed on a Zeiss Axioimager M2 System equipped with MBF Neurolucida Software (MBF) using 5x, 10x, or 20x objectives. ..

    Imaging:

    Article Title: Specific and comprehensive genetic targeting reveals brain-wide distribution and synaptic input patterns of GABAergic axo-axonic interneurons
    Article Snippet: Confocal imaging was performed on a Zeiss LSM 780 (CSHL St. Giles Advanced Microscopy Center) using ×10, ×20, or ×63 objectives. .. Imaging for grayscale overview images was performed on a Zeiss Axioimager M2 System equipped with MBF Neurolucida Software (MBF) using ×5, ×10, or ×20 objectives. ..

    Article Title: Dynamic regulation of autophagy during Semliki Forest virus infection of neuroblastoma cells
    Article Snippet: The secondary antibody, anti-rabbit AlexaFluor488, was diluted with blocking buffer and incubated at room temperature for 40 min. Coverslips were mounted using FluoroshieldTM with a DAPI mounting medium (Sigma-Aldrich, UK). .. Imaging of multiple z -axis positions was performed using a Zeiss AxioImager M2 system, and images were deconvoluted using the Zeiss Zen software deconvolution module. .. For quantification of LC3-positive punctae, images of only the DyLight 550 channel were selected at random and spots were counted in ImageJ.

    Article Title: Dynamic regulation of autophagy during Semliki Forest virus infection of neuroblastoma cells.
    Article Snippet: The secondary antibody, anti- rabbit AlexaFluor488, was diluted with blocking buffer and incubated at room temperature for 40 min. Coverslips were mounted using FluoroshieldTM with a DAPI mounting medium (Sigma- Aldrich, UK). .. Imaging of multiple z- axis positions was performed using a Zeiss AxioImager M2 system, and images were deconvoluted using the Zeiss Zen software deconvolution module. ..

    Article Title: Specific and comprehensive genetic targeting reveals brain-wide distribution and synaptic input patterns of GABAergic axo-axonic interneurons
    Article Snippet: Confocal imaging was performed on a Zeiss LSM 780 (CSHL St. Giles Advanced Microscopy Center) using 10x, 20x, or 63x objectives. .. Imaging for grayscale overview images was performed on a Zeiss Axioimager M2 System equipped with MBF Neurolucida Software (MBF) using 5x, 10x, or 20x objectives. ..



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    HG promotes mitochondrial dysfunction in ARPE-19 cells, accompanied by a decrease in TRAP1 levels. ( A ) Cell viability of ARPE-19 cells cultured with different glucose concentrations for 4 days. (n=4) ( B ) Cell viability of ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=4) ( C ) Intracellular ROS levels in cells cultured with 50mM glucose for 1–6 days. (n=3) ( D ) Identification of Δψ m through JC-1 staining after culturing ARPE-19 cells with 50mM glucose for 6 days. Images captured under <t>fluorescence</t> <t>microscope</t> show red fluorescence representing polymer form, indicating intact Δψ m, and green fluorescence representing monomer form, indicating decreased Δψ m. Quantified data presented on the right. Scale bar=20μm. (n=3) ( E ) ARPE-19 cells cultured with 50mM glucose for 6 days and treated with Calcein AM (1X) and CoCl2 (1X). Images captured under fluorescence microscope shown on the left. Quantified data presented on the right. Scale bar=20μm. (n=3) ( F ) TEM images of mitochondrial ultrastructure in ARPE-19 cells cultured with 50mM glucose for 6 days and control ARPE-19 cells. Quantified data presented on the right. Scale bar=2μm. ( G ) Western blot analysis of TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( H ) Western blot analysis of intramitochondrial TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days after mitochondrial extraction. (n=3) ( I ) Quantified data corresponding to Figure ( G ). ( J ) qRT-PCR analysis of TRAP1 mRNA expression in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( K ) Quantified data corresponding to Figure ( H ). The error bars in the above histograms represent the mean±SD of independent experiments. ns P>0.05, *P<0.05, **P<0.01, ***P<0.001,****P<0.0001.
    Fluorescence Microscope Zeiss Axioimager M2, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HG promotes mitochondrial dysfunction in ARPE-19 cells, accompanied by a decrease in TRAP1 levels. ( A ) Cell viability of ARPE-19 cells cultured with different glucose concentrations for 4 days. (n=4) ( B ) Cell viability of ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=4) ( C ) Intracellular ROS levels in cells cultured with 50mM glucose for 1–6 days. (n=3) ( D ) Identification of Δψ m through JC-1 staining after culturing ARPE-19 cells with 50mM glucose for 6 days. Images captured under <t>fluorescence</t> <t>microscope</t> show red fluorescence representing polymer form, indicating intact Δψ m, and green fluorescence representing monomer form, indicating decreased Δψ m. Quantified data presented on the right. Scale bar=20μm. (n=3) ( E ) ARPE-19 cells cultured with 50mM glucose for 6 days and treated with Calcein AM (1X) and CoCl2 (1X). Images captured under fluorescence microscope shown on the left. Quantified data presented on the right. Scale bar=20μm. (n=3) ( F ) TEM images of mitochondrial ultrastructure in ARPE-19 cells cultured with 50mM glucose for 6 days and control ARPE-19 cells. Quantified data presented on the right. Scale bar=2μm. ( G ) Western blot analysis of TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( H ) Western blot analysis of intramitochondrial TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days after mitochondrial extraction. (n=3) ( I ) Quantified data corresponding to Figure ( G ). ( J ) qRT-PCR analysis of TRAP1 mRNA expression in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( K ) Quantified data corresponding to Figure ( H ). The error bars in the above histograms represent the mean±SD of independent experiments. ns P>0.05, *P<0.05, **P<0.01, ***P<0.001,****P<0.0001.
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    Average 90 stars, based on 1 article reviews
    axioimager m2 microscope - by Bioz Stars, 2026-10
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    Carl Zeiss axioimager m2 microscopy system
    HG promotes mitochondrial dysfunction in ARPE-19 cells, accompanied by a decrease in TRAP1 levels. ( A ) Cell viability of ARPE-19 cells cultured with different glucose concentrations for 4 days. (n=4) ( B ) Cell viability of ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=4) ( C ) Intracellular ROS levels in cells cultured with 50mM glucose for 1–6 days. (n=3) ( D ) Identification of Δψ m through JC-1 staining after culturing ARPE-19 cells with 50mM glucose for 6 days. Images captured under <t>fluorescence</t> <t>microscope</t> show red fluorescence representing polymer form, indicating intact Δψ m, and green fluorescence representing monomer form, indicating decreased Δψ m. Quantified data presented on the right. Scale bar=20μm. (n=3) ( E ) ARPE-19 cells cultured with 50mM glucose for 6 days and treated with Calcein AM (1X) and CoCl2 (1X). Images captured under fluorescence microscope shown on the left. Quantified data presented on the right. Scale bar=20μm. (n=3) ( F ) TEM images of mitochondrial ultrastructure in ARPE-19 cells cultured with 50mM glucose for 6 days and control ARPE-19 cells. Quantified data presented on the right. Scale bar=2μm. ( G ) Western blot analysis of TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( H ) Western blot analysis of intramitochondrial TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days after mitochondrial extraction. (n=3) ( I ) Quantified data corresponding to Figure ( G ). ( J ) qRT-PCR analysis of TRAP1 mRNA expression in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( K ) Quantified data corresponding to Figure ( H ). The error bars in the above histograms represent the mean±SD of independent experiments. ns P>0.05, *P<0.05, **P<0.01, ***P<0.001,****P<0.0001.
    Axioimager M2 Microscopy System, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/axioimager+m2+system/axio+microscope+observer+z1+%CE%B2/pmc12264458-82-11-10
    Average 90 stars, based on 1 article reviews
    axioimager m2 microscopy system - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

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    Carl Zeiss zeiss axioimager m2
    HG promotes mitochondrial dysfunction in ARPE-19 cells, accompanied by a decrease in TRAP1 levels. ( A ) Cell viability of ARPE-19 cells cultured with different glucose concentrations for 4 days. (n=4) ( B ) Cell viability of ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=4) ( C ) Intracellular ROS levels in cells cultured with 50mM glucose for 1–6 days. (n=3) ( D ) Identification of Δψ m through JC-1 staining after culturing ARPE-19 cells with 50mM glucose for 6 days. Images captured under <t>fluorescence</t> <t>microscope</t> show red fluorescence representing polymer form, indicating intact Δψ m, and green fluorescence representing monomer form, indicating decreased Δψ m. Quantified data presented on the right. Scale bar=20μm. (n=3) ( E ) ARPE-19 cells cultured with 50mM glucose for 6 days and treated with Calcein AM (1X) and CoCl2 (1X). Images captured under fluorescence microscope shown on the left. Quantified data presented on the right. Scale bar=20μm. (n=3) ( F ) TEM images of mitochondrial ultrastructure in ARPE-19 cells cultured with 50mM glucose for 6 days and control ARPE-19 cells. Quantified data presented on the right. Scale bar=2μm. ( G ) Western blot analysis of TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( H ) Western blot analysis of intramitochondrial TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days after mitochondrial extraction. (n=3) ( I ) Quantified data corresponding to Figure ( G ). ( J ) qRT-PCR analysis of TRAP1 mRNA expression in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( K ) Quantified data corresponding to Figure ( H ). The error bars in the above histograms represent the mean±SD of independent experiments. ns P>0.05, *P<0.05, **P<0.01, ***P<0.001,****P<0.0001.
    Zeiss Axioimager M2, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    zeiss axioimager m2 - by Bioz Stars, 2026-10
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    Carl Zeiss zeiss m2 axioimager
    HG promotes mitochondrial dysfunction in ARPE-19 cells, accompanied by a decrease in TRAP1 levels. ( A ) Cell viability of ARPE-19 cells cultured with different glucose concentrations for 4 days. (n=4) ( B ) Cell viability of ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=4) ( C ) Intracellular ROS levels in cells cultured with 50mM glucose for 1–6 days. (n=3) ( D ) Identification of Δψ m through JC-1 staining after culturing ARPE-19 cells with 50mM glucose for 6 days. Images captured under <t>fluorescence</t> <t>microscope</t> show red fluorescence representing polymer form, indicating intact Δψ m, and green fluorescence representing monomer form, indicating decreased Δψ m. Quantified data presented on the right. Scale bar=20μm. (n=3) ( E ) ARPE-19 cells cultured with 50mM glucose for 6 days and treated with Calcein AM (1X) and CoCl2 (1X). Images captured under fluorescence microscope shown on the left. Quantified data presented on the right. Scale bar=20μm. (n=3) ( F ) TEM images of mitochondrial ultrastructure in ARPE-19 cells cultured with 50mM glucose for 6 days and control ARPE-19 cells. Quantified data presented on the right. Scale bar=2μm. ( G ) Western blot analysis of TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( H ) Western blot analysis of intramitochondrial TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days after mitochondrial extraction. (n=3) ( I ) Quantified data corresponding to Figure ( G ). ( J ) qRT-PCR analysis of TRAP1 mRNA expression in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( K ) Quantified data corresponding to Figure ( H ). The error bars in the above histograms represent the mean±SD of independent experiments. ns P>0.05, *P<0.05, **P<0.01, ***P<0.001,****P<0.0001.
    Zeiss M2 Axioimager, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    HG promotes mitochondrial dysfunction in ARPE-19 cells, accompanied by a decrease in TRAP1 levels. ( A ) Cell viability of ARPE-19 cells cultured with different glucose concentrations for 4 days. (n=4) ( B ) Cell viability of ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=4) ( C ) Intracellular ROS levels in cells cultured with 50mM glucose for 1–6 days. (n=3) ( D ) Identification of Δψ m through JC-1 staining after culturing ARPE-19 cells with 50mM glucose for 6 days. Images captured under fluorescence microscope show red fluorescence representing polymer form, indicating intact Δψ m, and green fluorescence representing monomer form, indicating decreased Δψ m. Quantified data presented on the right. Scale bar=20μm. (n=3) ( E ) ARPE-19 cells cultured with 50mM glucose for 6 days and treated with Calcein AM (1X) and CoCl2 (1X). Images captured under fluorescence microscope shown on the left. Quantified data presented on the right. Scale bar=20μm. (n=3) ( F ) TEM images of mitochondrial ultrastructure in ARPE-19 cells cultured with 50mM glucose for 6 days and control ARPE-19 cells. Quantified data presented on the right. Scale bar=2μm. ( G ) Western blot analysis of TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( H ) Western blot analysis of intramitochondrial TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days after mitochondrial extraction. (n=3) ( I ) Quantified data corresponding to Figure ( G ). ( J ) qRT-PCR analysis of TRAP1 mRNA expression in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( K ) Quantified data corresponding to Figure ( H ). The error bars in the above histograms represent the mean±SD of independent experiments. ns P>0.05, *P<0.05, **P<0.01, ***P<0.001,****P<0.0001.

    Journal: Clinical Ophthalmology (Auckland, N.Z.)

    Article Title: TRAP1 Improves Diabetic Retinopathy by Preserving Mitochondrial Function

    doi: 10.2147/OPTH.S521660

    Figure Lengend Snippet: HG promotes mitochondrial dysfunction in ARPE-19 cells, accompanied by a decrease in TRAP1 levels. ( A ) Cell viability of ARPE-19 cells cultured with different glucose concentrations for 4 days. (n=4) ( B ) Cell viability of ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=4) ( C ) Intracellular ROS levels in cells cultured with 50mM glucose for 1–6 days. (n=3) ( D ) Identification of Δψ m through JC-1 staining after culturing ARPE-19 cells with 50mM glucose for 6 days. Images captured under fluorescence microscope show red fluorescence representing polymer form, indicating intact Δψ m, and green fluorescence representing monomer form, indicating decreased Δψ m. Quantified data presented on the right. Scale bar=20μm. (n=3) ( E ) ARPE-19 cells cultured with 50mM glucose for 6 days and treated with Calcein AM (1X) and CoCl2 (1X). Images captured under fluorescence microscope shown on the left. Quantified data presented on the right. Scale bar=20μm. (n=3) ( F ) TEM images of mitochondrial ultrastructure in ARPE-19 cells cultured with 50mM glucose for 6 days and control ARPE-19 cells. Quantified data presented on the right. Scale bar=2μm. ( G ) Western blot analysis of TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( H ) Western blot analysis of intramitochondrial TRAP1 in ARPE-19 cells cultured with 50mM glucose for 1–6 days after mitochondrial extraction. (n=3) ( I ) Quantified data corresponding to Figure ( G ). ( J ) qRT-PCR analysis of TRAP1 mRNA expression in ARPE-19 cells cultured with 50mM glucose for 1–6 days. (n=3) ( K ) Quantified data corresponding to Figure ( H ). The error bars in the above histograms represent the mean±SD of independent experiments. ns P>0.05, *P<0.05, **P<0.01, ***P<0.001,****P<0.0001.

    Article Snippet: Fluorescence images were captured using a Zeiss AxioImager M2 fluorescence microscope (Zeiss, Germany) with the following parameters: Oxidized state (green fluorescence): Excitation wavelength of 488 nm, emission filter of 500–550 nm;- Reduced state (red fluorescence): Excitation wavelength of 581 nm, emission filter of 590–650 nm.Images were analyzed using Zen 3.1 software and quantified with ImageJ software.

    Techniques: Cell Culture, Staining, Fluorescence, Microscopy, Polymer, Control, Western Blot, Extraction, Quantitative RT-PCR, Expressing